My IVF incubator is late….now what?

Categories: Practice Tips
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Published on: April 10, 2018

By Jon Schmidt (Trans Ova Genetics)

An unfortunate reality with IVF is the occasional need to utilize commercial shipping companies in the transportation of oocytes to and embryos from the IVF lab.  Many of us who work with IVF shipments have experienced a delayed, lost, or cold incubator.  These are unfortunate events that can be catastrophic to results and end in frustrated lab staff, transfer teams, and clients.  Below are a few suggestions on how to handle incubators that are compromised in transit while embryos are going back to the practitioner or client’s farm.

d0 = OPU day
d1 = fertilization day
d7 = normal transfer day

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Evidence-Based ET: Where does the transferred embryo have to be placed in order to maximize pregnancy rate?

Categories: Evidence-Based ET
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Published on: March 13, 2014

Note: This is the fourth in the series of topics suggested by the AETA Board of Directors. Should any readers want to suggest future topics, please email them to me.

John F. Hasler

Jfhasler05@msn.com

Where does the transferred embryo have to be placed in order to maximize pregnancy rate?

Well, we know where a bovine embryo belongs when it first travels through the UT junction!  Initially, it will be in the tip of the uterine horn, and if the embryo resulted from fertilization of the animal’s own ovulated oocyte, then the embryo will be in the horn on the same side (ipsilateral) as the CL. However, that is not necessarily where the embryo will end up and implant.  Embryo migration within the uterus is well-known in litter producing domestic animals, such as swine, but it also has been shown to occur in single ovulating cattle at a frequency of 2 to 3% (for review see McMillan and Peterson, 1999), although Scanlon (1972) reported no trans-uterine migration in more than 600 single ovulating cows. He did, however, note that a series of previous published reports indicated a migration percentage of 0.3 to 1.6%. Migration has not been reported in cattle following the non-surgical transfer of a single embryo transferred to the horn either to the ipsilateral or contralateral to the ovary with the CL, although the sample sizes were small. (more…)

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