Effect of superstimulation on the expression of microRNAs and genes involved in steroidogenesis and ovulation in Nelore cows

Categories: Research Publications
Tags: No Tags
Comments: Comments Off
Published on: February 18, 2021

P H Santos 1R A Satrapa 2P K Fontes 1F F Franchi 1E M Razza 1F Mani 3M F G Nogueira 4C M Barros 1A C S Castilho 5

1Universidade Estadual Paulista (UNESP), Institute of Biosciences, Campus Botucatu, Department of Pharmacology, Botucatu, São Paulo, Brazil.

2Universidade Federal do Acre (UFAC), Center of Biological and Natural Sciences, Rio Branco, Acre, Brazil.

3Universidade Estadual Paulista (UNESP), Institute of Biosciences, Campus Botucatu, Department of Chemistry and Biochemistry, Botucatu, São Paulo, Brazil.

4Universidade Estadual Paulista (UNESP), School of Sciences and Languages, Campus Assis, Department of Biological Sciences, Assis, São Paulo, Brazil.

5Universidade Estadual Paulista (UNESP), School of Sciences and Languages, Campus Assis, Department of Biological Sciences, Assis, São Paulo, Brazil. Electronic address: anthony@unoeste.br.

https://pubmed.ncbi.nlm.nih.gov/29407901/

Theriogenology 2018 Apr 1;110:192-200. doi: 10.1016/j.theriogenology.2017.12.045. Epub 2018 Jan 11.

Abstract

To better understand the impact of ovarian superstimulation on bovine follicular microenvironment, Nelore cows (Bos taurus indicus) were subjected to ovarian superstimulation with follicle stimulating hormone (FSH, n = 10; P-36 protocol) or FSH combined with eCG (n = 10; P-36/eCG protocol). Follicular fluid was analyzed for cholesterol concentration. Granulosa cells were analyzed by RT-qPCR to assess the expression of genes involved in steroidogenic and ovulatory and expression of microRNAs involved in final follicular development and luteinizing hormone/choriogonadotropin receptor (LHCGR) expression. Plasma concentration of estradiol was also measured. Follicular fluid from the P-36 group showed higher concentration of cholesterol than that of control (non-superstimulated) cows. Plasma concentration of estradiol was higher in the P-36/eCG group. Abundance of STAR and FSHR mRNAs were lower in granulosa cells from the P-36/eCG group. In contrast, LHCGR mRNA abundance was higher in superstimulated granulosa cells from the P-36 group and showed a pattern opposite to that of miR-222 expression. Ovarian superstimulation did not affect the expression of other markers (mmu-miR-202-5p, has-miR-873, has-miR-144, and their target genes, CREB, TGFBR2, and ATG7) of antral follicle development. However, the mRNA expression of VEGF pathway components was modulated by P-36 treatment. Taken together, these results demonstrate that superstimulatory protocols modify steroidogenic capacity, increase plasma estradiol, and regulate the abundance of VEGF system, LHCGR mRNA and suppress the expression of miR-222 in bovine granulosa cells.

Comments are closed.

Welcome , today is Friday, June 18, 2021