‘There is only one thing that is truly important in an IVF laboratory: everything’ Cairo Consensus Guidelines on IVF Culture Conditions

Categories: Research Publications
Tags: No Tags
Comments: Comments Off
Published on: February 18, 2021

Cairo Consensus Group

https://www.sciencedirect.com/science/article/pii/S1472648319307540#!

Reproductive BioMedicine Online Volume 40, Issue 1, January 2020, Pages 33-60

Abstract

This proceedings report presents the outcomes from an international expert meeting to establish consensus guidelines on IVF culture conditions. Topics reviewed and discussed were: embryo culture – basic principles and interactions; temperature in the IVF laboratory; humidity in culture; carbon dioxide control and medium pH; oxygen tension for embryo culture; workstations – design and engineering; incubators – maintaining the culture environment; micromanipulation – maintaining a steady physcochemical environment; handling practices; assessment practices; culture media – buffering and pH, general composition and protein supplementation, sequential or single-step media for human embryo culture; use and management – cold chain and storage; test equipment – calibration and certification; and laboratory equipment and real-time monitoring. More than 50 consensus guideline points were established under these general headings.

Keywords: Culture conditions, Incubators, IVFMaintenance, Medium, Quality control

Comments are closed.

Welcome , today is Monday, March 1, 2021