Comment on requirements for donors of embryos exporting USA to Australia

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Published on: July 6, 2018

by Pat Comyn
AETA Education Committee Chair

I recently collected embryos from a donor for export of those embryos to Australia. As part of the visit, I collected both an EDTA tube and a red top blood tube for submission for BVD (Bovine Pestivirus) testing in keeping with section 4 (below) of the Australian embryo import requirements.

I wanted to do an ELISA due to lower cost and turn around being faster compared to the VI. However, I had not heard of an ELISA for BVD being done on peripheral WBC (Buffy coat) and suspected that the description of the ELISA test in the IREGs was in error (the Australia IREGs was updated 4/18). After consultation with Pat Phillips, DVM (Hawkeye Breeders – embryo exporter), David Duxbury, DVM (AETA Government Liaison Committee Chair) and their inquiries to APHIS – Madison WI, we found that BVD ELISA on whole unclotted blood was being done at Indiana Animal Disease Diagnostic Laboratory / Purdue University College of Veterinary Medicine. The results of the whole blood ELISA are acceptable for export to Australia. The client savings for the buffy coat ELISA were considerable as compared to viral isolation and the turn around was excellent (one day VS 2 weeks).

Section 4 is below.

4Bovine pestivirus

Prior to the export of this consignment of embryos each female donor gave a negative result to one of the following tests for bovine pestivirus:

  • an antigen-capture enzyme-linked immunosorbent assay (ELISA) on peripheral blood leucocytes


  • a virus isolation test on blood or serum

[The veterinary certificate must indicate the option that applies. The table must include dates of sampling for test, type of tests used, test results.]

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