Food for FSH Thought: Inductions of Superovulation Using Several FSH Regimens In Holstein-Fresian Heifers

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Published on: June 17, 2016


Yoshiyuki TAKAHASHI and Hiroshi KANAGAWA

(Received for publication, February 2, 1985)

Eighty-three Holstein-Friesian virgin heifers were randomly divided into four groups and superovulated with one of the four FSH regimens as follows: Group 1) twice daily injections of a constant dose of 5 mg of FSH for a period of 4 days, giving a total dose of 40 mg in 8 fractions; Group II) as in Group I with additional 5 mg of FSH on the 5th day, giving a total dose of 45 mg; Group ill) twice daily injections on a decreasing dose regimen of 5, 4, 3 and 2 mg successively for 4 days with the addition of 2 mg of FSH once on the 5th day, giving a total dose of 30 mg; Group N) similar to Group ill, but a mixture of FSH and LH in the ratio of 5: 1 was used, giving a total dose of 30 mg of FSH and 6 mg of LH in 9 fractions. Nonsurgical embryo recovery was performed 7 or 8 days after estrus. Percentage of good embryos in Group II was significantly greater than that in Group I (P < O. 05). There was no significant difference between Groups II and ill in any of the criteria analyzed. Group N was superior to the other groups in superovulation rate and embryo yielding. Significantly greater numbers of total eggs and good embryos were observed in Group N compared to those in Groups I and II (P<0.05). Percentage of the heifers with more than 3 good embryos in Group N was significantly higher than that in Groups I, II and ill (P < O. 05).

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