AETA President’s Report – Fall 2019

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Published on: October 11, 2019

Greetings to you on behalf of AETA.

The 2019 Annual AETA CETA Convention is now weeks away and we are extremely excited about its venue, atmosphere, program, entertainment, and much more we have planned for you and your colleagues. The convention committee, lead by Matt Dorshorst, DVM, has done an outstanding job.

We thank all of the many volunteers that contribute to the success of AETA. For example, Pat Comyn, DVM, and his education committee, continue to provide this quarterly newsletter to
keep members abreast on current news and educational materials that enhance our embryo transfer services.

AETA, along with AABP and AASRP, provided two amazing seminars this summer for its members on bovine and small ruminant embryo transfer, which continues to be in high demand for its top quality educational opportunity it provides.

Social media and the internet continue to be key areas to communicate and we invite you to like AETA on Facebook to follow news and posts from our industry. As you are well aware, the internet also provides occasional challenges from outside hackers that promote “phishing”
schemes to try to solicit funds from individuals and businesses. As always, use good common sense with emails that look suspicious.

AETA is extremely proud of our student scholarship program and we plan to award several deserving students again this year. We also had a terrific response to the first annual poster contest and we encourage you to interact with the authors who attend the convention.

From great organizations in industry stem inspiring people and leaders, and AETA is no exception, so look forward to some exciting presentations this fall, including the Edwin Robertson Lifetime Achievement Award.

The last week of October is gearing up to be a fun and exciting time with friends and colleagues! We hope your travel plans are complete. We look forward to seeing everyone in beautiful Colorado Springs!

Sincerely:
Matt Iager, DVM
President AETA 2019

AETA Annual Meeting: Final Program Available

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Published on: October 11, 2019

Save the date for the 2019 AETA-CETA/ACTE Annual Meeting!

The 2019 AETA-CETA/ACTE will be held from October 24–26, 2019, in Colorado Springs, Colorado. The conference will be held at the Cheyenne Mountain Resort.

All of the AETA scientific, social, and exhibitor information can be found on the AETA Annual Convention. The final program has been added!

Colorado Springs is a wonderful city to hold this convention. Below you will find some links to help you plan your stay.

Garden of the Gods
Pikes Peak Region
Olympic Training Center
Cheyenne Mountain Zoo

Manitou Springs

This is a very small list of the many attractions that Colorado Springs has to offer. Please check out Visit Colorado Springs to customize your stay.

We look forward to seeing you all in beautiful Colorado in October!

Sincerely,

AETA

2019 AETA Candidates for the Board of Directors

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Published on: October 11, 2019
Larry Lanzon, DVM

Born and raised in Detroit, Michigan, Larry Lanzon graduated from Michigan State University School of Veterinary Medicine in 1980. He has spent 39 years in private practice, the last 3 years with Embryo Inc. Lanzon joined the AETA in 1982 and was privileged to have Edwin Robertson, Bob Rowe, Reuben Mapletoft, and Joe Wright as his mentors when getting started in assisted reproductive technology. Lanzon earned a masters of preventative veterinary medicine in epidemiology in 2013 from UC Davis.

Lanzon and his wife Cathy raised three children—Jesse, Casey, and Caitlin—together in Turlock, which is absolutely his proudest accomplishment. Presently, there are three grandsons—Casey, Cody, and Charlie—in the Lanzon clan.

Through the years, Lanzon spent his time with the Boy Scouts of America; attending swim meets, water polo games, and gymnastic meets; raising Red Angus cattle; and fly-fishing with his children.

In practice, Lanzon enjoyed the diversity of dairy practice, working with and teaching students from all over the world. However, he quickly developed an interest in assisted reproductive technology and has continued to learn, teach, and enjoy embryo transfer and IVF. He respectfully thanks all those who helped him along the way.

Brad Lindsey, PhD

In 1984, Brad Lindsey helped start and manage the former Granada Corporation’s equine services division. In 1988, he transferred to their bovine embryo transfer division and managed their main production lab and was involved in commercializing IVF services. In 1992, he was hired by Trans Ova Genetics in Sioux Center, Iowa, to start their IVF service division. In 1994, Lindsey began his PhD with Jim Kinder at the University of Nebraska–Lincoln. His research focus was endocrine regulation of reproductive hormones in beef cattle. In 1997, he went to Rockhampton, Queensland, Australia, to pursue postdoctoral research at the former Tropical Beef Centre with Michael D’Occhio.

In 1998, he was hired as general manager of Stroud Veterinary Embryo Services in Weatherford, Texas, under the direction of Brad Stroud. Between 2000 and 2005, Lindsey worked for AB Technology (now ABT360), Minitube of America, and Genetic Resources Int’l (now Sexing Technologies) in various technical roles, directing research and product development and developing an IVF lab service platform. In 2005, he started Rex Consulting, providing ET services and technical support to cattle producers, biotech companies, and other ET firms, and in 2008 incorporated as Ovitra Biotechnology Inc. Ovitra continues to provides commercial and contract ET services, technical support, consulting, and training to beef and dairy producers, research universities, and other ET firms.

Dr. Lindsey is currently an active member, serving on several committees within AETA (Certification and Research), IETS (HASAC, Manuals and Forms), and the Texas Southwest Cattle Raisers Association (Research and Education). He and his wife, Mary, live in Midway, Texas, and are active in their church and community. They have two grown children, Grace Richardson and Payton Lindsey.

Greg Schueller, DVM

Greg Schueller grew up on a dairy farm in southwest Wisconsin, where he developed his interest in and passion for dairy cattle and veterinary medicine. He received his DVM from the University of Wisconsin in 1991.

Upon graduation, he joined a mixed-animal practice in southwest Wisconsin, where he focused on dairy reproduction and herd health from 1991 to 2009. He added embryo transfer services to the practice in 1998 and joined the AETA at that time. In 2006, he became AETA certified and continued to grow the ET side of the practice until he joined Sunshine Genetics, an embryo transfer exclusive practice, in 2009. Currently, he is an owner of Sunshine Genetics with his business partner Aaron Prososki.

His wife Marcia is a speech therapist in the Fort Atkinson school district. They have three wonderful daughters, Brianna (20), Kailyn (18), and Melia (15). In his spare time, he enjoys traveling with his wife and family and is beginning to look forward to the next phase of life as his children are reaching adulthood and gaining independence.

Cary Schroeder, DVM

Cary Schroeder is co-owner of Lena Veterinary Clinic, an L0 doctor mixed-animal practice in Lena, lllinois. Schroeder received his BS in animal science in 1980 and DVM degree in 1984 from lowa State University. He has been a member of AETA since 2000 and certified in embryo transfer since 2003. He is currently serving on the AETA audit committee.

He and his wife Sarah, their children John (Abby), Christin (Dana Keefer), and Casey (Brianna), and their six grandchildren all live in or near Lena, lllinois. They enjoy spending time with their family, boating, traveling, hunting, and golfing.

Reminder: Proposed By-Laws Changes to be Voted on at AETA Annual Business Meeting–October 25, 2019

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Published on: October 11, 2019

Dear AETA members:

The purpose of AETA is to unite those organizations and individuals in the United States engaged in the embryo transfer industry into an affiliated federation operating under self- imposed standards of performance and conduct. As AETA delivers on education and standards of quality through its certification program, we become the “voice” for our diverse membership.

Based on recommendations from the membership, the board has proposed the following modifications to the By-Laws relating to membership categories. The reasons for the changes are several. These changes will make official some membership categories that have been previously offered but not officially recognized in the By-Laws, such as Life Membership. It will also make official reduced membership fees to former student members for one year following graduation. The proposed changes will also move the Association in the direction of a professional association and away from a trade association. We feel this is important to continue to foster relationships with other professional veterinary and animal science organizations such as AABP, AASRP, and IETS. This transition will allow the Association to continue to fulfill its primary purpose of education and promoting a high standard of quality through certification. Finally, it will reduce some redundancy in the membership categories by combining Regular and Associate Membership into only Associate Membership.

• Professional: An individual who is actively engaged in the embryo transfer industry who is a licensed veterinarian in the US or holds a PhD in reproductive physiology from a US institution. Other equivalent degrees as approved by the Board of Directors. Professional Members are eligible to vote, serve on committees, and hold an office in the Association.

• Associate: Organizations or individuals engaged in a business or occupation related to the embryo transfer industry that do not meet the requirements for Professional, Emeritus, Life, or Student Memberships. These Associate Members may not vote but will otherwise be entitled to full privileges of membership and can attend meetings, serve on committees, and receive newsletters.

• Life: Board of Directors can confer, at its discretion, honorary Life Membership to a practitioner for exemplary service. Life Members may enjoy the rights of their previous Professional Membership status.

• Emeritus: Individuals who have been Professional Members of the Association for a period of at least 10 years and who deem themselves as retired from activities associated with Professional Membership. Emeritus Members may enjoy the rights of their previous Professional Membership status. Emeritus Membership is granted by an application, in writing, to the Board of Directors, who have the sole right to invoke or revoke the Emeritus status to a Professional Member.

• Student: Individuals enrolled in an academic program at the graduate level and pursuing either a veterinary degree or PhD in reproductive physiology. Academic status must be verified annually by an academic advisor or a Professional Member. Student Members are not eligible to vote or hold office in the Association. Following graduation, former Student Members are eligible to become Professional Members at half of the cost of the Professional Membership for one year only.

This change in membership classification simplifies our current method and makes it easily distinguishable with less redundancy.

This is to inform you of a By-Laws change that will be proposed at the 2019 annual convention in Colorado Springs, Colorado, October 24-26, 2019. We hope you understand and can identify with this proposal. Please reach out to board members with questions you may have.

Sincerely,
AETA Board of Directors

AETA_Bylaws_Proposed_Changes

Remember When? AETA Past Meeting History

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Published on: October 11, 2019

Fast Facts:

  • At the organizational meeting of the AETA, 29 August, 1981, in Denver :
    • First order of business was an agreement to thoroughly study the problem of existing ET patents and pending litigation against ET practitioners
  • 1981 AETA Annual Dues = $1.00 per embryo handled ($250 minimum and $5,000 maximum)
  • The first Board met the first time for one day in Sept. 1982 at the O’Hare Hilton
  • In addition, the AETA asked for and received donations for a legal fund

1983 – First Convention 

  • Location: Ft. Collins, Colorado
    • Hotel cost was $33/night
  • Dates: January 18-19, 1983 – following the IETS conference
  • There were 51 attendees
  • The program included:
    • Established 4 standing committees
    • Panel presentation on problems with export of embryos
    • Augspurger patent lawsuit review
  • Did you know? Bob Garey, Executive Vice President of the AETA was hit over the head in his motel room by an intruder who entered at night through a sliding door
1983 Proceedings Cover
(more…)

ICAR Announces Dr. Gabriel Bó as Recipient of 2020 Simmet Prize for Assisted Reproduction

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Published on: October 11, 2019

Sydney, Australia, October 3, 2019: The International Congress on Animal Reproduction (ICAR) announces that Dr. Gabriel Bó of the Instituto de Reproducción Animal Córdoba (IRAC) in Córdoba, Argentina is the recipient of the 2020 Simmet Prize for Assisted Reproduction. The prize, which is the most prestigious award in animal reproduction and one of the largest of its kind, was given for the pioneering efforts of Dr. Bó to develop practical protocols for fixed-time artificial insemination, superovulation and embryo transfer in cattle.

Dr. Bó is currently President and Director of Research and Post-graduate training at IRAC and Professor of Obstetrics and Biotechnology of Reproduction at the Veterinary School of the Instituto de Ciencias Basicas y Aplicadas, Universidad Nacional de Villa Maria in Cordoba, Argentina. IRAC is one of the few organizations of its kind with missions in research, teaching, and clinical service including embryo transfer, artificial insemination, and gamete and embryo cryopreservation. Over 1300 veterinarians in South and Central America have received education on animal reproduction since IRAC opened in 1995.

Bó and his colleagues have developed what are now the de facto standard protocols for superovulation in cattle. Research conducted by him and his collaborators on estrus synchronization and fixed-time artificial insemination has been one of the key drivers for implementation of these technologies by producers throughout South America and globally. Together, these practices are revolutionizing genetic selection and reproductive management of cattle.

The Simmet Prize is sponsored by Minitube International and administered by ICAR. The prize, established as a memorial to the accomplishments of Dr. Ludwig Simmet, a pioneer in development of artificial insemination in farm animals and founder of Minitube, recognizes an active research scientist for basic and applied research published during the previous six years in the area of assisted reproduction of animals. The prize is presented each 4 years and includes an award of 50,000 euros.

Cryptorchidism

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Published on: October 11, 2019

Written by Dr. Pat Comyn

I have recently had a client ask me what his options were with a holstein bull calf of very high genetic value (genomic prediction) that happened to be a unilateral cryptorchid. Aside from a grunt, I didn’t know how to answer, so I thought I should educate myself. As it turns out, the causes of crytorchidism in cattle aren’t  very well understood. A some observations from some reading.

  1. The left testicle is most commonly affected.
  2. Male repro tract development occurs from a different tissue (wolffian duct which is part of the mesonephros developing into the epididymis, vas deferens, seminal vesicle, and ejaculatory duct) than the female tract (Müllerian duct which differentiates into vagina, cervix, uterus etc).
  3. While many argue that chryptorchidism is “heritable”, the heritability of this trait is not well characterized meaning that we don’t know what percent of the development of cryptorchid syndrome is truly genetic and what percent is environmental (meaning uterine / maternal hormonal influence).
  4. This is an excerpt from Cryptorchidism and associated problems in animals1 R. P. Amann2 and D. N. R. Veeramachanen: Animal Reproduction and Biotechnology Laboratory Colorado State University, Fort Collins, CO 80523-1683 USA.

“Early reports on cryptorchidism (e.g., de Graaf, 1668) provided evidence of two or more diseases, because undescended testes are not located at a common non-scrotal site. Nevertheless, the general perception had been that cryptorchidism is a single disease with moderate heritability, incomplete penetrance, expressed only in males (sex specific expression), and concentrated by inbreeding or minimized by culling affected males and all siblings. However, the notion of a single-locus gene problem gave way to acceptance of a polygenic recessive model, based on relatively small studies with pigs (Sittmann and Woodhouse, 1977; Rothschild et al, 1988) and dogs (Cox et al, 1978; Nielen et al., 2001); also data for men (Czeizel et al., 1981). It is evident that abnormalities in >20 genes are associated with human cryptorchidism (Klonisch et al., 2004) and, currently it is accepted that cryptorchidism has many causes including genetic, epigenetic, and environmental components.”

  1. A search on line showed no studies where back breeding of cryptorchids to dam or siblings had been done to characterize heritability coefficient.
  2. A unilateral cryptorchid will on average produce 60 – 80% the spermatozoa of a normal bull.
  3. The affected testicle should be removed so not to place abnormal spermatozoa in the ejaculate. Too, removal will enhance hypertrophy of the normal testicle.

So here we are. A unilateral cryptorchid dairy bull calf. The owner vents his / her frustration and also inquires as to your thoughts on how to proceed. Here are my thoughts…

  1. If a dairy bull and high enough genomics, offer him out. There are dairy bulls in collection now that are unilateral cryptorchids.
  2. Consider private CSS EU qualified collection then see if the semen can be purchased by a bull stud and sold.
  3. Like point B except the producer sells the semen.
  4. The money might not be as good as a normal bull purchase but one can make lemonade from lemons.

Articles of Interest

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Published on: October 11, 2019

https://phys.org/news/2018-03-bovine-embryos-early-human.html

https://www.animal-reproduction.org/current

https://epigeneticsandchromatin.biomedcentral.com/articles/10.1186/s13072-017-0171-z

https://www.ncbi.nlm.nih.gov/pubmed/28131573

AETA President’s Report – Summer 2019

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Published on: July 26, 2019

Greetings fellow AETA members:

As the summer heat settles in, we wish everyone a safe and relaxing season. The year tends to pass quickly, and we continue to gear up for our annual convention in beautiful Colorado Springs. We thank Dr. Matt Dorshorst and his convention planning team for their outstanding efforts on this convention. You can be certain we have planned a special event for all of you!

Our research committee has added a poster contest for all AETA and CETA members to participate in, including students. This will spice up our exhibitor area for additional interaction and educational opportunities. Up to 10 students will receive complimentary registration as an incentive to participate. We will continue to award our student scholarship winners as well.

AETA has teamed up with AASRP to host a small ruminant embryo transfer seminar at Ohio State University in June, and we continue to provide the Edwin Robertson Embryo Transfer Seminar at Virginia Tech with AABP in August. We have enjoyed collaborating with other allied partners in our industry to provide the best educational opportunities in the business.

IETS has invited AETA and CETA to a joint convention in 2023. We will fully analyze the schedule and logistics of this concept for that calendar year to decide whether the opportunity works well for each of the organizations involved. Please contact us with your comments and suggestions.

We hope you continue to appreciate the efforts of our promotion and membership committees. We completed a new brochure for use at AABP and AASRP seminars and conventions as well as other events throughout the year. Facebook has attracted a lot of attention from users who “like” our page and follow the news and highlights of our association. The AETA brand has been promoted nationally and internationally.

AETA has a lot to celebrate, and our committees are vibrant and innovative. We invite you to participate and serve as well as provide feedback to us throughout the year so we can steadily communicate with members. Thank you for the opportunity to serve this great organization, and we look forward to visiting with you in Colorado this fall.

Matt Iager, DVM

Proposed By-Laws Changes to be Voted on at AETA Annual Business Meeting–October 25, 2019

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Published on: July 26, 2019

Dear AETA members:

The purpose of AETA is to unite those organizations and individuals in the United States engaged in the embryo transfer industry into an affiliated federation operating under self- imposed standards of performance and conduct. As AETA delivers on education and standards of quality through its certification program, we become the “voice” for our diverse membership.

Based on recommendations from the membership, the board has proposed the following modifications to the By-Laws relating to membership categories. The reasons for the changes are several. These changes will make official some membership categories that have been previously offered but not officially recognized in the By-Laws, such as Life Membership. It will also make official reduced membership fees to former student members for one year following graduation. The proposed changes will also move the Association in the direction of a professional association and away from a trade association. We feel this is important to continue to foster relationships with other professional veterinary and animal science organizations such as AABP, AASRP, and IETS. This transition will allow the Association to continue to fulfill its primary purpose of education and promoting a high standard of quality through certification. Finally, it will reduce some redundancy in the membership categories by combining Regular and Associate Membership into only Associate Membership.

  • Professional: An individual who is actively engaged in the embryo transfer industry who is a licensed veterinarian in the US or holds a PhD in reproductive physiology from a US institution. Other equivalent degrees as approved by the Board of Directors. Professional Members are eligible to vote, serve on committees, and hold an office in the Association.
  • Associate: Organizations or individuals engaged in a business or occupation related to the embryo transfer industry that do not meet the requirements for Professional, Emeritus, Life, or Student Memberships. These Associate Members may not vote but will otherwise be entitled to full privileges of membership and can attend meetings, serve on committees, and receive newsletters.
  • Life: Board of Directors can confer, at its discretion, honorary Life Membership to a practitioner for exemplary service. Life Members may enjoy the rights of their previous Professional Membership status.
  • Emeritus: Individuals who have been Professional Members of the Association for a period of at least 10 years and who deem themselves as retired from activities associated with Professional Membership. Emeritus Members may enjoy the rights of their previous Professional Membership status. Emeritus Membership is granted by an application, in writing, to the Board of Directors, who have the sole right to invoke or revoke the Emeritus status to a Professional Member.
  • Student: Individuals enrolled in an academic program at the graduate level and pursuing either a veterinary degree or PhD in reproductive physiology. Academic status must be verified annually by an academic advisor or a Professional Member. Student Members are not eligible to vote or hold office in the Association. Following graduation, former Student Members are eligible to become Professional Members at half of the cost of the Professional Membership for one year only.

This change in membership classification simplifies our current method and makes it easily distinguishable with less redundancy.

This letter is to inform you of a By-Laws change that will be proposed at the 2019 annual convention in Colorado Springs, Colorado, October 24-26, 2019. We hope you understand and can identify with this proposal. Please reach out to board members with questions you may have.

Sincerely,

AETA Board of Directors

Save the date for the 2019 AETA-CETA/ACTE Joint Annual Convention!

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Published on: July 26, 2019

The 2019 AETA-CETA/ACTE Joint Annual Convention will be held from October 24–26, 2019, in Colorado Springs, Colorado. The conference will take place at the Cheyenne Mountain Resort.

All of the AETA scientific, social and exhibitor information can be found on the AETA Annual Convention page as it becomes available. Check back often!

Colorado Springs is a wonderful city to hold this convention. Below you will find some links to help you plan your stay.

Garden of the Gods
Pikes Peak Region
Olympic Training Center
Cheyenne Mountain Zoo

This is a very small list of the many attractions that Colorado Springs has to offer. Please check out Visit Colorado Springs to customize your stay.

We look forward to seeing you all in beautiful Colorado in October!

Sincerely,

AETA

Recap: AETA/AASRP 2019 Small Ruminant Embryo Transfer Seminar

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Published on: July 26, 2019

The AETA/AASRP 2019 Small Ruminant Embryo Transfer Seminar was held June 19 to 22, 2019, at The Ohio State University Large Animal Services satellite veterinary teaching hospital in Marysville, Ohio. The meeting was organized by Dr. Eric Gordon.

The course started on Wednesday, June 19, with a review of small ruminant reproductive physiology by Dr. Sherri Clark. Dr. Bill Croushore and Dr. Dave Dixon discussed embryos processing, grading, and cryopreservation. Drs. Mattes and Shipley discussed embryo collection, anesthesia, and sync methods as well as more reproductive physiology and handling of semen. Later in the day, a goat was surgically flushed as a demonstration.

On Thursday, June 20, and Friday, June 21, course participants broke out into teams of three and flushed three goats or sheep each day. All flushing occurred under gas anesthesia. After each flush, the teams searched embryos, and viable embryos were cryopreserved. Later in the day, Dr. Shipley discussed semen collection and cryopreservation. He also elaborated on reproductive physiology. On Saturday, June 22, teams of three practitioners each laparoscopically inseminated three ewes.

The meeting went very well, and it is felt that participants were quite satisfied with the value of this course. Dr. Eric Gordon at OSU CVM at Marysville deserves a huge thank you for his efforts in bringing this course together and in convincing clients to provide animals to flush. Dr. Justin Kieffer with OSU Animal Science was a huge help in bringing in his technician staff and in assisting with planning for animal usage in this CE meeting and for providing, via the animal science department, some of the animals used.

Preliminary trials of a specific gravity technique in the determination of early embryo growth potential†

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Published on: July 26, 2019

Read full article here

S.D. Prien,1,2,* C.E. Wessels,2 and L.L. Penrose1

. 2015 Sep; 30(9): 2076–2083.
Published online 2015 Jul 22. doi: 10.1093/humrep/dev178
PMCID: PMC4542720
PMID: 26202920

Abstract

STUDY QUESTION

Can a modified specific gravity technique be used to distinguish viable from nonviable embryos?

SUMMARY ANSWER

Preliminary data suggests a modified specific gravity technique can be used to determine embryo viability and potential for future development.

WHAT IS KNOWN ALREADY

Single embryo transfer (SET) is fast becoming the standard of practice. However, there is currently no reliable method to ensure development of the embryo transferred.

STUDY DESIGN, SIZE, DURATION

A preliminary, animal-based in vitro study of specific gravity as a predictor of embryo development using a mouse model.

PARTICIPANTS/MATERIALS, SETTING, METHODS

After a brief study to demonstrate embryo recovery, experiments were conducted to assess the ability of the specific gravity system (SGS) to distinguish between viable and nonviable embryos. In the first study, 1-cell mouse embryos were exposed to the SGS with or without previous exposure to an extreme heat source (60°C); measurements were repeated daily for 5 days. In the second experiment, larger pools of 1-cell embryos were either placed directly in culture or passed through the SGS and then placed in culture and monitored for 4 days.

MAIN RESULTS AND THE ROLE OF CHANCE

In the first experiment, viable embryos demonstrated a predictable pattern of descent time over the first 48 h of development (similar to previous experience with the SGS), while embryos that were heat killed demonstrated significantly altered drop patterns (P < 0.001); first descending faster. In the second experiment, average descent times were different for embryos that stalled early versus those that developed to blastocyst (P < 0.001). Interestingly, more embryos dropped through the SGS developed to blastocyst than the culture control (P < 0.01).

LIMITATIONS, REASONS FOR CAUTION

As this is a preliminary report of the SGS technology determining viability, a larger embryo population will be needed. Further, the current in vitro study will need to be followed by fecundity studies prior to application to a human population.

WIDER IMPLICATIONS OF THE FINDINGS

If proven, the SGS would provide a noninvasive means of assessing embryos prior to transfer after assisted reproductive technologies procedures, thereby improving fecundity and allowing more reliable SET.

STUDY FUNDING/COMPETING INTEREST(S)

The authors gratefully acknowledge the funding support of the U.S. Jersey Association, the Laura W. Bush Institute for Women’s Health and a Howard Hughes Medical Institute grant through the Undergraduate Science Education Program to Texas Tech University. None of the authors have any conflict of interest regarding this work.

TRIAL REGISTRATION NUMBER

none.

Keywords: embryo development, embryo selection, embryo viability, specific gravity, buoyance, noninvasive, zygote, blastocyst

Legality of Compounded Estradiol for Embryo Transfer

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Published on: July 25, 2019

The AABP office has received questions from members regarding the legality of using compounded estradiol products in cattle for embryo transfer protocols. AABP has also been in discussion with the FDA about the use of compounded estradiol products in food animals. Compounding from approved drugs in animals is only permitted under the narrowly defined conditions outlined in AMDUCA (Section 21 CFR 530.13). To be permitted, extralabel use from compounding of approved animal drugs or approved human drugs must be in compliance with all relevant provisions of 21 CFR 530 (AMDUCA), including the provisions limiting extralabel use to treatment modalities when the health of an animal is threatened or suffering or death may result from failure to treat. The extralabel use regulation also does not provide for compounding from active pharmaceutical ingredients (APIs or bulk drugs—i.e., the raw chemical) for use in animals. Therefore, it is illegal for veterinarians to use or prescribe compounded estradiol for cattle, or any form of estrogenic compounds for production purposes, including embryo transfer and synchronization protocols. AABP encourages cattle veterinarians to refrain from administering or prescribing compounded estradiol for the following reasons:

  • AMDUCA only allows for extralabel drug use when the health of an animal is threatened. There is no production allowance, particularly for compounding; therefore one cannot use human-approved drugs (e.g., ECP, Pfizer) or a different form of an animal-approved drug (e.g., growth-promoting implants) for production purposes.
  • Compounding from a bulk product is specifically prohibited in AMDUCA regulations.
  • The safety, potency, efficacy, stability, sterility, and disposition of compounded products is unknown. Compounded products do not undergo FDA inspection, potency testing, or efficacy testing. Veterinary compounding pharmacies that also compound for humans are under federal regulation and are FDA inspected; however, this only applies to the human side of the compounding operation. Veterinary compounding pharmacies do not have this level of oversight. There is no guarantee of the safety or efficacy of compounded products, and liability for the use of such products falls on the veterinarian in the event of an adverse reaction or violative residue.
  • Because the safety, efficacy, potency, and disposition of the compounded product is not known, it is impossible to assign a withdrawal interval for compounded products.
  • The use of compounded products in food animals places a veterinarian at risk of professional liability.

The need for estradiol for successful embryo transfer protocols has not been unequivocally established. For example, data from nearly 7,000 collections did not demonstrate a difference when using GnRH in place of estradiol in the protocol.1 Additional references are available on the Reproduction Committee page of the website at https://aabp.org/members/Reproduction.asp. Veterinarians who engage in federally prohibited activities put themselves at risk and also risk the profession’s reputation for appropriate and judicious oversight of pharmaceutical products in our cattle patients. This is especially of concern when using an unapproved and illegally manufactured hormone product.

AABP Newsletter 5 May 2019.

Please contact Dr. Fred Gingrich at fred@aabp.org with any questions.

Submitted by the AABP Reproduction Committee and the AABP Committee on Pharmaceuticals and Biologics.

1Hinshaw, R.H. Comparison of GnRH and estradiol 17β for follicle turnover in bovine superovulation protocols. Proceedings of the American Embryo Transfer Association 2013, p. 15.

In vitro culture systems: how far are we from optimal conditions?

Categories: Research Publications
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Published on: July 25, 2019

C. Wrenzycki

http://dx.doi.org/10.21451/1984-3143-AR869

Anim Reprod, vol.13, n3, p.279-282, 2016

Abstract

Over the past decades in vitro production (IVP) of bovine embryos has been significantly improved. Nevertheless, embryos generated in vitro still differ from their in vivo produced counterparts. Embryos must adjust to multiple microenvironments at preimplantation stages. Consequently, maintaining or mimicking the in vivo situation in vitro will aid to improve the quality and developmental competence of the resulting embryo.

cattle, embryo, in vitro production

AETA President’s Report – Spring 2019

Categories: President's Message
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Published on: April 17, 2019

By Dr. Matt Iager, 2019 AETA President

Greetings to you from AETA. As spring business gears up, don’t forget to take time to interact with friends and family and thank the farmers and ranchers who provide the avenue for our services.

AETA has been busy focusing and planning for a fun and successful 2019! In January, IETS hosted a great conference in New Orleans and expressed their gratitude to affiliate organizations, such as ours. AETA is strongly poised as a leader in our industry. We are very fortunate in our current financial position, goals for our organization, membership involvement, and relationships to allied industries. However, with every organization, we realize our fruitfulness was achieved by many hard-working individuals working for the good of each member, so complacency is never an option.

In March, our Board of Directors met at the convention site in Colorado Springs for our winter board meeting. We made plans for the year and are excited about the many achievements made by each committee, along with ideas to inspire new progress for our committees. We are also very proud and thankful for the many AETA members that volunteer their time and talents to serve these important roles. It’s never too late to volunteer, so please reach out to me or the committee chairs to let us know your interests.

Our social media has been extremely active with over 1,500 Facebook “likes” on the AETA page and over 7,000 individuals being reached in the last 30 days about our posts and promotion. Be sure to “like” AETA on Facebook! We also hope you enjoy the advertising in national breed magazines and also the exposure on the Internet, via webcast, blogs, and news posts from various organizations, all of which continue to brand AETA’s relevance and importance in today’s technology.

The convention committee is finalizing topics and speakers for the convention. You can be sure this convention will be special and memorable. The social events will take place at the resort, which is incredibly tasteful, with a lake, beach area, and Cheyenne Mountain directly adjacent to the resort hotel. The research committee is hosting a poster contest at this year‘s convention, so be sure to look for more information about this new opportunity!

We look forward to the 2019 AETA & CETA joint annual convention, October 24–26, in beautiful Colorado Springs. I would like to thank all of you for the opportunity to serve AETA and look forward to seeing you in Colorado!

Save the date for the 2019 AETA-CETA/ACTE Annual Meeting!

Categories: Annual Meeting
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Published on: April 17, 2019

The 2019 AETA-CETA/ACTE will be held from October 24–26, 2019, in Colorado Springs, Colorado. The conference will be held at the Cheyenne Mountain Resort.

All of the AETA scientific, social, and exhibitor information can be found on the AETA Annual Convention page as it becomes available. Check back often!

Colorado Springs is a wonderful city to hold this convention. Below you will find some links to help you plan your stay.

Garden of the Gods
Pikes Peak Region
Olympic Training Center
Cheyenne Mountain Zoo
Manitou Springs

This is a very small list of the many attractions that Colorado Springs has to offer. Please check out Visit Colorado Springs to customize your stay.

We look forward to seeing you all in beautiful Colorado in October!

Sincerely,

AETA

AETA Small Ruminant Ovum-Pick-Up Brief

Categories: Practice Tips
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Published on: April 17, 2019

Submitted by Dr. Kevin Lindell

Recently, Dr. Rachael Gately, Tufts Veterinary Field Service, had the opportunity (through a collaborative research project) to explore the realm of small ruminant ovum pick-up (OPU)/in vitro fertilization (IVF).

We thought it would be interesting to the membership to relay our initial experiences and challenges.

To date, donors have been collected after superstimulation, using protocols somewhat similar to those we use in cattle. Without prior manipulation of the estrus cycle, a SR CIDR is placed on day −7. On day 0 donors receive PG and 1.5 cc of follicle-stimulating hormone (FSH) AM/PM; day 1 donors receive 1.0 cc of FSH AM/PM and then 1.0 cc of FSH on the morning of day 2. Oocyte collection is performed on day 4, resulting in a coasting period (time between the last FSH injection and time of collection) of approximately 48 hours. Variables such as FSH dosage, number of total injections, and coasting period seemed to make a significant impact on follicular recruitment, size, and competency, although the data set is very small to this point.

Oocyte collection is performed under general anesthesia using a short-needle system. A threaded small ruminant 18-ga OPU needle, attached directly to aspiration tubing (without using a metal rod) has been a relatively simple and successful method for oocyte collection, in conjunction with our regular aspiration/vacuum pump. Although we would ultimately like to offer small ruminant OPU as a purely laparoscopic procedure, it has been difficult to stabilize the ovaries well enough, so we have instead opted to externalize the ovaries briefly for aspiration.

As this procedure seems to be growing in interest amongst our clientele, we are looking forward to additional small ruminant oocyte collection trials and embryo development results.

The abstract below from a recent review article was also a useful tool as we initially organized equipment, consumables, and donor protocols.

Theriogenology (86) 2016

Recent advances in in vitro embryo production in small ruminants

By Maria-Teresa Paramio*, Dolors Izquierdo

ABSTRACT

To increase productivity in the small ruminant industry, the genetic material of these species should be improved. In vitro embryo production could be an important technology to reach this goal by combining selected male and female gametes. In the world, marketing of in vitro-produced embryos is an economical activity which is progressing rapidly in cattle but is practically nonexistent in small ruminants. Since the birth of the first lamb and kid using IVF in the 80s, several studies have been carried out; however, results still are inconsistent and unpredictable. Moreover, significantly fewer research groups are working on embryo production in small ruminants than in cattle and pigs. Although conventional methodologies of oocyte IVM, IVF, and IVC in small ruminants give rise to blastocysts, significant variation exists between experiments. One important reason for these differences is the heterogeneity of the pool of oocytes recovered from ovaries from slaughtered females. Oocyte quality, also referred to as competence, is the key factor in the success of in vitro embryo production programs. Different criteria are used to select the best oocytes for fertilization, such as follicle size, oocyte diameter and morphological appearance, and Brilliant Cresyl Blue staining. New research lines aimed at improving oocyte competence are: (1) arresting nuclear maturation in vitro allowing optimal capacitation of cytoplasm, (2) growing oocytes inside the follicle, and (3) identification of biomarkers of oocyte competence in granulosa and cumulus cells and metabolites in the follicular fluid.

Practice Tip – Battery Backup

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Published on: April 17, 2019

Submitted by Dr. Pat Comyn

This jump starter has come in really handy for those times when I am breeding or placing embryos in a place with no power. With all the moisture we have had in my area in the last year (92 inches), it is sometimes really difficult to get a truck near the working area. Also, trucks near the working area = dented trucks. So this gadget is great for keeping the thaw bath warm. It also charges cell phones.  

 

Comparison of iSperm to current accepted methods for raw semen analysis

Categories: Evidence-Based ET
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Published on: April 17, 2019

Download the poster

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